Month: August 2020

Fruit of the Holy Spirit explained

Bible stories : Fruit of the Holy Spirit? The most important concept regarding the fruit of the Spirit is that it is the Spirit’s fruit. We are often admonished to be more loving, to have joy, or to be kind, but we cannot do so without the power of the Holy Spirit in us. It is the Holy Spirit who gives love (1 John 4:7), joy (1 Thessalonians 1:6), peace (Romans 8:6), patience (Colossians 1:11), kindness (2 Peter 1:1-9), goodness (2 Thessalonians 1:11), faith (Ephesians 2:8-9), gentleness (2 Corinthians 10:1), and self-control (2 Peter 1:6). Still, we do have a part. Our attitude and our actions affect how the Holy Spirit works in us. We can quench His power and influence (Ephesians 4:30), or we can share in the work of sanctification (1 Peter 1:15; Hebrews 12:14). The “fruit” of the Holy Spirit is directed and powered by God. If we do not abide in Christ, allowing the Holy Spirit to bear fruit in us, we will be clipped off and tossed away just like a shriveled vine (John 15:1-6).

Galatians 5:22-23: 22 But the fruit of the Spirit is love, joy, peace, patience, kindness, goodness, faithfulness, 23 gentleness, self-control; against such things there is no law. The fruit of the Spirit is what grows in us as born-again believers and helps us to show the love of God to those who don’t know Him. The closer we grow to God, the more of the fruit that becomes evident in our lives.

The “Fruit of the Spirit” is a great passage of scripture for kids that immediately gives them something they understand and can relate to-fruit! This series of lessons gives you a great opportunity to include activities, crafts, songs and games revolving around cheerful, colorful objects. Purple balloons (grapes) and other vibrant colors can be part of your visual representation of the fruits. Fake fruits are often available in craft stores for additional props.

The primary key to everything. Along with temperance (self-control), love is a bookend that helps hold the other fruit in place. It is a love that surpasses human understanding and causes a person to be filled with the fullness of God (Ephesians 3:18-19). Its divine characteristics are detailed in 1 Corinthians 13:4-8. “Love is patient, love is kind. It does not envy, it does not boast, it is not proud. It is not rude, it is not self-seeking, it is not easily angered, it keeps no record of wrongs. Love does not delight in evil but rejoices with the truth. It always protects, always trusts, always hopes, always perseveres. Love never fails”.

Self-control; Moderation or self-restraint in action or statement; It is control over your entire being (body, soul and spirit). A person who has self-control is mild and calm, avoids extreme behavior, and exercises self-restraint in both actions and speech. After all, temperance and love are the bookends that hold all the other fruit in place. So now you know what exactly Jesus meant when he said in Matthew 7:20, “Wherefore by their fruits you shall know them.”

The lessons we offer are written on a level that children can fully understand. Paired with our coloring pages and additional activities the lessons can bring this group of attributes to life. Check out our selection of children’s songs to add additional fun and enthusiasm to learning about the fruit of the Spirit. See more information on the Fruit of the Holy Spirit video on YouTube.

The fruit of the Spirit is the natural result of the presence of the Holy Spirit residing in a believer. Philippians 2:13 says, “for it is God who works in you, both to will and to work for his good pleasure.” Conversely, Isaiah 64:6 says, “all our righteous deeds are like a polluted garment…” In and of ourselves, we cannot do good. As Hebrews 13:20-21 says, “Now may the God of peace … equip you with everything good that you may do his will.” God accomplishes this as the Holy Spirit indwells believers, changing their character (Philippians 1:6) and manifesting good “fruit.” Galatians 5:22-23 lists the characteristics of this fruit.

Let’s talk about The Parable Of The Workers In The Vineyard

Let’s talk about The Parable Of The Workers In The Vineyard? But Jesus challenges that expectation. The special inheritance that Israel believes to be hers is going to be extended—in full—to other nations. It’s not that the Hebrews were against God extending blessings to other nations. They just always assumed that God would value those nations below Israel. In response to the kingdom of God, Israel would make the same argument as the early laborers, “you have made them equal to us who have borne the burden of the work.”

Jesus often uses parables to reveal what the kingdom of heaven is like. He portrays how one enters the kingdom and who the different characters are. In this Parable of the Laborers or Workers in the Vineyard, there are things that He tells the disciples and us about the grace of God and that God is always more than fair. Here is a discussion on this parable and what Jesus means in giving it.

The master of the house would seem to be God and the vineyard is the place where those servants who have been called to work for the master as laborers will enter into the work. The laborers are those who have been called and saved by God. They enter into the work or their calling by God under the guidance of the master, which is Jesus Christ. In another place in the Scriptures, Jesus uses this symbolism of believers being used by God to labor for the Lord as in Matthew 9:37-38 where He says “The harvest is plentiful, but the laborers are few; therefore pray earnestly to the Lord of the harvest to send out laborers into his harvest.”

But such an interpretation raises more questions. Are we free to knowingly live a life of sin, as long as we make that last confession before death? The parable seems to support an odd loophole to salvation, that getting into heaven is decided by a technicality of whether your sins have been forgiven rather than that you love God and want to spend eternity with Him.

Jesus spent a great deal of His ministry announcing the coming of God’s kingdom and overcoming first-century presumptions by teaching people to recognize that kingdom. And a lot of His parables focused on communicating valuable truths about this kingdom. One misunderstanding that Jesus needed to clarify was the idea that the Jews held a special insider relationship with God. From the very beginning, God told Abraham that all the nations of the world would be blessed through his offspring (Genesis 22:18), but as far as the Israelites were concerned, these other nations would never be as blessed as they were. Discover extra details with the The Parable Of The Workers In The Vineyard video on YouTube.

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See top movies free online? The darkness is all-consuming, as is despair over a lost past and future, and a purgatorial present, in Vitalina Varela, Pedro Costa’s aesthetically ravishing true tale of its protagonist, a Cape Verde resident who returns to Portugal mere days after her estranged husband’s death. Vitalina wanders through this dilapidated and gloomy environment, which Costa shoots almost exclusively at night, the better to conjure a sense of ghosts navigating a dreamscape of sorrow, suffering and disconnection. Each of the director’s images is more ravishing than the next, and their beauty – along with an enveloping soundscape of squeaking beds, sheets blowing in the wind, and rain pattering on crumbling roofs – is enchanting. Presenting its story through fractured plotting and dreamy monologues, the Portuguese master’s latest is a series of tableaus of lovelorn grief concerning not only Vitalina but also an aged priest in spiritual crisis and another young man poised to endure his own tragedy. The film’s formal grandeur – its compositional precision, and painterly interplay of light and dark – is overwhelming, as is the majestic presence of Vitalina herself.

The tony Pennsylvania prep school in which Tayarisha Poe’s nimble debut takes place might bring to mind mean-rich-kid chronicles like Cruel Intentions — but it has more in common with Rian Johnson’s 2005 baby-faced neo-noir Brick. Selah and the Spades is a teen drama in which the line between social clique and mob family feels incidental, taking place in a boarding-school bubble that’s enthralling and insular, privilege serving as a kind of leveling agent that makes day-to-day skirmishes for dominance the only thing that matters. And at the still center of this surprisingly tumultuous world is Selah (Lovie Simone), a character whose desire for a successor wars with her instinct to destroy anyone who challenges her place — even when it’s someone of her own choosing. It’s a compelling portrait of someone who, having made herself the queen of this limited kingdom, finds herself terrified of life when she leaves.

Disciplined in its approach and unapologetic about its contrivances, Ben Affleck’s basketball coach in crisis drama The Way Back is a sports movie that understands the fundamentals. What it lacks in flashiness or ingenuity — the underdog narrative of a crappy team hitting its stride under the leadership of a gruff coach hits all the requisite Hoosiers notes — it makes up for with an oddly enthralling downbeat craftsmanship. Little details, like the freeze-frame when the scores of games pop up on screen or the click-clack percussion-heavy music, accumulate emotional power over the film’s brisk runtime. Playing a washed-up ex-athlete with an immediately apparent drinking problem and a number of strategically hidden personal demons, Affleck delivers a weary performance that resonates with his off-screen persona (and his recent tabloid headlines) in ways both obvious and surprising. In brief stretches, director Gavin O’Connor, who helmed the similarly intense melodramas Miracle and Warrior, pulls off the ultimate sports movie trick of making you believe the character’s redemption isn’t inevitable. Every win is a battle — even if you know the results going in. See extra info at watch tv shows online.

Jean-Pierre and Luc Dardenne gaze into the dark heart of religious fanaticism in Young Ahmed, a drama that’s all the more chilling for proffering no easy answers. By the time the filmmakers’ story begins, urban 13-year-old Ahmed (newcomer Idir Ben Addi) has already been indoctrinated by a jihad-encouraging imam (Othmane Moumen). No amount of adult counter-programming can affect the kid, and when he attacks a female teacher (Myriem Akheddiou) for her modernist Islamic teachings, he winds up in a juvenile detention center and, then, at a farm where the affections of Louise (Victoria Bluck) complicate his worldview. With a stony countenance and dark eyes that mask his interior thoughts, Ahmed is a chilling protagonist in thrall to a rigid ideology that preaches violence against all heretics. Their handheld camerawork trailing him as he embarks on his cataclysmic rise-and-fall journey, the directors’ aesthetics are as formally rigorous and evocative as ever, capturing the unyielding nature of zealotry, as well as the difficulty of loosening extremism’s terrible grip on individuals’ hearts and minds.

Whenever we start any discussion of the best media players and how great they are, the name VLC automatically comes at the top. But, why is it so? What’s the reason behind its massive popularity? VLC Media Player is a free and open-source media player that’s available for all popular platforms. Created by the VideoLAN project, VLC Media Player is known to support a vast number of audio and video compression methods and file formats. Over the years, VLC has earned the reputation of being the “play everything” video player. VLC is one of the best PC media players due to its fast and simple nature that doesn’t involve any painstaking steps. The software also features different customization options that can help you improve your viewing experience and change the look of the player. It’s also often used a streaming media player. This amazing Windows Media Player alternative also receives frequent updates and has a large user community. It runs on all Windows versions, from Windows 10 to Windows XP SP3. From time-to-time, this free movie player keeps receiving new features and updates. You can also check out our dedicated article on VLC tricks.

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Best disposable gloves online shopping

Best disposable gloves online shopping? A distinctive advantage of our active ingredient, not shared by alcohol-based antiseptics or hydrogen peroxide antiseptic, is that it does not cause a burning sensation when applied to broken skin. Instead, it is actually used as a wound cleaner. Why do we stand behind our active ingredient? The compound is especially useful for its antimicrobial activity and is an active ingredient in many useful consumer products. SAFTEY: Our product can be applied on the skin safely, however, remember to keep it away from your eyes.

Another factor “is that culturally, the U.S. wasn’t really prepared to wear masks,” unlike some countries in Asia where the practice is more common, said Chin-Hong. Even now, some Americans are choosing to ignore CDC guidance and local mandates on masks, a hesitation that Chin-Hong says is “foolhardy.” Rutherford was more blunt. The legitimate concern that the limited supply of surgical masks and N95 respirators should be saved for health care workers should not have prevented more nuanced messaging about the benefits of masking. “We should have told people to wear cloth masks right off the bat,” he said.

Cleaning is the removal of germs and dirt from surfaces, but cleaning does not kill germs. It merely removes germs and lowers the risk of spreading infection. Disinfecting is the process of using chemicals to kill germs on surfaces. This doesn’t mean dirty surfaces are cleaned or germs are removed, but by killing germs on a cleaned surface, disinfecting lowers the risk of spreading infection even more. “Wear disposable gloves when cleaning and disinfecting,” Bronstein said. “This will reduce the spread of germs from your hands to other areas of your house, car, or groceries.”

Exclusive 47 PCS Survival Kit – Uniquely customized by U.S military veterans, our survival kit includes 28pcs emergency survival gears, 16pcs medical supplies, 10pcs fishing tools and 4 in 1 Molle EMT pouch to suit all your needs. Includes folding knife, muols to better suit your needs. The all in one survival kit includes tactical folding knife, wire saw, pen, fire starter, compass, emergency blanket, folding pliers,flashlight, tactical defense whistle, saber card, bottle clip, carabiner, etc. Discover more info at smartresponder.info emergency kit.

Professional carpet cleaning is the very best way to give your carpets the cleaning they deserve. Professionals are the only people who have the proper truck-mounted equipment to get the job done right. They use powerful hot water extraction that goes beyond the carpet, cleaning the backing and padding for a more comprehensive clean-up. This is the most recommended method for the best maintenance. Solvents: For marker, ink, or oil stains, it is best to use some kind of solvent (even WD 40 can work). Always remember to blot, don’t rub. Let the solvent sit for half an hour and then remove it with soapy water. For pet stains, you may have to find an enzyme remover.

A facemask can be used to make a fashion statement. The standard protective masks are, of course, a standard white or blue. However, if you’re not in a situation in which you are providing healthcare services, but just need to cover up while out in public, there’s no end to the possibilities for exotic prints, bright colors, and various glamorous add-ons (think sequins). If you’ve mastered the art of constructing your own, you don’t have to worry about using a practical material, because you can make new ones rather than having to wash them so frequently. Your creation may even become the source of “mask envy” in people who marvel at your ingenuity.

SmartResponder.info is your best health store. Be prepared and be safe! A first responder is a person with specialized training who is among the first to arrive and provide assistance at the scene of an emergency, such as an accident, natural disaster or terrorist attack. First responders typically include paramedics, medical emergency technicians, police, firefighters, rescuers and other trained members of organizations involved in this type of work. Discover additional details at web for first responders.

Car engine remaps and vehicle repair Berkshire from mot-centre.com

Fast car engine remaps and vehicle repair Reading by mot-centre.com? The ECU of your car is like its brain. For your car to deliver it’s best performance you have to keep it in a fit condition at all times. This is the only way to ensure that you get the best in both driving as well as handling. Modern day cars do not have manual controls. Nowadays sophisticated technology is being incorporated in the form of computerized controls that guide and ensure Engine performance.

You cannot drive a car and not know about the essentials. Check your coolant levels, brake, wiper fluid, fluid, and keep track of them. Do not forget to load them up to avoid future problems. Auto repairs are costly but you can completely avoid them when you take care of your car. It doesn’t hurt to consult a mechanic right away when you notice that something is not right. There may be an out of pocket cost for you but that is better than spending thousands of dollars on costly auto repairs. When you see a mechanic, any potential problem will be detected by him and he will be able to provide a solution right away.

Most car owners underestimate how important car battery maintenance is. Although your battery will run out of juice eventually, keeping up with it will extend the life. If you want your car battery to run smoothly, you need to make sure it has a good connection. Residue can build up and prevent your tire from starting. You can get battery-cleaning fluid at most auto parts stores. Removing the battery and cleaning the connections will extend the life and improve the connection.

How do we change the parameters in the software? We work with leading-edge and efficient programs for the mapping and changing of the engine software. These programs allow us to detect all necessary parameters and change them accordingly. The knowledge and experience in handling this technical issue play a decisive part here. As mentioned above, we leave the component protection of the engine and the peripherals, e.g. the turbocharger, activated. You can see how the map of the boost looks like here. By changing the duty cycle, we can increase or lower the boost. Even very small changes can have a big impact, so special caution is absolutely necessary here! If the boost is too high, the turbocharger might speed excessively and disintegrate into pieces. It is awfully important to know which bolt to turn in order to get the desired result.

Is the technician trained on your specific vehicle make? Cars and trucks today are extremely complex machines, and their unique characteristics vary heavily from brand to brand. Making certain that your technician has obtained the proper training for your specific vehicle is crucial since special tools and procedures — many of which are not easily available to the “average” auto mechanic — are required to correctly service your auto.

ECU Repairs and Engine Remap Reading, Ecus being a electrical part on your vehicle can fail from time to time but we are experts in saving you a lot of money by repairing your existing Ecu because buying a replacement ecu can be very very expensive so we have invested a lot of time effort and money being able to repair your Ecu saving you lots of money in the process. The performance of modern vehicles are limited by the manufacturers for many reasons. One being that they have to allow for the use of poor quality fuels and infrequent servicing but not least of all it allows manufactures the ability to introduce performance increases throughout a vehicles production life. See additional info on Remapping Service Reading.

How much does an MOT cost? The Vehicle and Operator Services Agency sets the maximum fee official test centres can charge for an MOT. It’s currently £54.85 for cars and motor caravans and £29.65 for motorbikes, but many garages charge less than this – sometimes up to 50% less. Search online for “cheap MOT” or “MOT discount” to find out how you can save money on your car’s next MOT. Top 5 simple reasons for cars failing the MOT test: Nearly two in five MOT tests are a fail first time. Yet often this is because of minor faults the owner could easily have fixed beforehand.

Warning signs: Do not take general warning signs like clunking, knocking or abnormal vibrations lightly. These could well be early signs of a potentially big problem. However, always remember, these tips are meant to help you keep your car in top shape in the period between professional services.

Have you ever collected your car after an MOT, and then been left open mouthed at the cost? Some MOT centres advertise one price and then add on hidden charges. There are no hidden charges with Reading Service & MOT Centre. The price you see is the price you pay. We have an outstanding reputation for great customer service, high quality repairs and low prices. Call us today for a quote, and find out why. Great value MOT testing, at times to suit you.

The engine can be tuned for conditions that you set, like better fuel, removing or raising the rev limiter, increasing boost in turbo charged engines, increasing or decreasing air to fuel ratios for more horse power. Tuning can remove barriers that can limit the benefits of other modifications to an engine. It can also be a good value for horsepower per dollar spent. Find more info on this website.

Website design for auto dealers

Website design for auto dealers? Getting any local businesses to rank high on Google is becoming increasingly more difficult, but one of the most competitive and complex industries of local SEO is car dealership. Nowadays, car shoppers do a lot of their research online before they even enter a dealership showroom. (And many people don’t even know what kind of car they want when they start searching.) According to Google, the average car shopper only visits two dealerships when they’re looking for a new vehicle. This means that it is even more important than ever for car dealerships to rank higher on local search results.

Inbound link authority is the single biggest ranking factor (>60% of Google’s algorithm!) because it provides basic understanding to a search engine on how to value a website. Having a backlink on a very credible site can be a huge boost compared to several hundred coming from less authoritative sites.

With our innovative technology, clean design, and industry leading support, we guarantee your success on the DealersGear platform. Providing industry leading support is our biggest commitment to you. Our knowledgeable and friendly staff are here to help you every step of the way. Digital Marketing that sells cars. With all conversions measured on your website we will guarantee you results. After years of running our own dealerships, we wanted to bring to the market what we didn’t have. With our data driven approach to all social media and search engines channels, we will go above and beyond to find what works and what doesn’t. From our Facebook ads, to PPC management, to YouTube advertising, and utilizing our top converting website, we will make sure your sales and services are up. Discover additional details on website design for car dealers.

Local SEO is an ideal way to promote your business’ visibility on location-based searches. It helps your business get found online by local prospects. This type of SEO becomes exceptionally important if you have a business that provides a service in a specific locality. According to HubSpot, 72% of people who did a local search visited a store within 5 miles. It shows how effective local SEO is.

Why Local SEO matter? Local SEO helps people to easily find your local business online. It helps your business to become highly visible online, and rank high in local SERPs making it easier for customers to find you. What is the future of local search marketing? With more and more people using smartphones, iPads, etc., and voice search usage on the rise, the future lies in local mobile marketing. Having an SEO-compliant website and a strong local SEO strategy will get your website on to Google’s local listing pack.

DealersGear is the first all-in-one, marketing solution that was built by dealers for dealers. This solution enables dealers to manage their digital presence, manage their inventory, and manage their websites from one central command center. Our company provides products from multi media management, inventory management, intelligent Auto dealers websites, posting tools to Craigslist, Facebook, and everything else in between. See more information on this website.

Shayna Davidov Hanson or the rise of a real estate & wellness professional

Who is Shayna Davidov Hanson and some of her achievements? Shayna and her team of experts at SDHanson and Associates, are constantly educating themselves on new developments and up and coming construction in Miami, in order to provide cutting edge info to her clients before it hits the market. S.D. Hanson and Associates can help you with up and coming projects such as Faena & The Edition on Mid Beach, Ritz Carlton Residences in Miami Beach, Glass South Beach, The One on South Beach, Surfside Four Season Richard Meier/Surf Club, Chateau Beach in Surfside, 250 Wynwood near Miami’s hot Design District, Chateau Ocean in Sunny Isles, Porsche Tower, Aqualina, The Mansions at Seven Townhomes in Bal Harbour, The Bath Club Estates in Miami Beach, Kai Bay Harbour Islands, The Flatiron Brickell, SLS Luxe Brickell, Park Grove in Coconut Grove …and many more.

Shayna Davidov Hanson about herself: Let me be your Real Estate and lifestyle coach– and help you arrive at your “next level” in life, by combining strategies that incorporate the powerful intersection between financial and life goals. Nothing gives me greater pleasure for helping clients grow their portfolios to maximize their return on investment, as well as quality of life.

Shayna Davidov Hanson about find & register your building’s carbon footprint and plan safe “Re-entry” for inhabitants & workers- with Arc : More and more, consumers are demanding BOTH stringent attention to environmental health standards as well as desire built environments that are committed to sustainability. With the Arc Re-entry tools, decision makers and owners can provide both! Some of units of metrics for Arc Certification scorecards include: water usage, occupancy inputs and electricity usage, amongst other things. There is a different set of metrics from Energy Star to Arc. Energy Star is solely based on source energy. Arc, however, uses measurements for Greenhouse Gas emissions — GHGs. That said, Arc Software can integrate with Energy Star programs. Arc simply provides a more in-depth overview of scoring related to emissions, carbon footprint and overall building air and health.

The real estate market in Miami is an ever changing landscape of opportunities and new neighborhoods as well as existing are serviced by SDHanson and Associates. Shayna has been a top producer in the prevalent and luxurious Miami Beach real estate marketplace, however has increasingly transacted on properties from Sunny Isles Beach to Coconut Grove and Hallandale. Single family home communities with waterfront properties such as Bay Harbour Islands, the Sunset Islands, Star Island, Coral Gables, and Venetian Islands are plentiful in South Florida as are luxury condominiums in Brickell, Miami Beach, Bal Harbour, and Hollywood. The SDHanson and Associates offers premier service in these established markets as well as consultation on emerging neighborhoods such as the Design District, Wynwood, and Edgewater. Discover additional info on Shayna Davidov Hanson.

Shayna Davidov Hanson about WELL : Socially, it’s only responsible thing to do to start pulling in available technologies and techniques in building and community design- to ensure water, air and living systems are healthy and available. In both the residential space and commercial space, consumers are seeking more attention into healthy design and healthy spaces. McKinsey & Company predicts “Wellness is the next trillion dollar industry as employers and developers invest in healthy living programs and as customers take more responsibility for optimizing their own health.”

Bifurcation analysis studies

Mitotic checkpoint regulation learning? Combinatorial complexity is a central problem when modeling biochemical reaction networks, since the association of a few components can give rise to a large variation of protein complexes. Available classical modeling approaches are often insufficient for the analysis of very large and complex networks in detail. Recently, we developed a new rule-based modeling approach that facilitates the analysis of spatial and combinatorially complex problems. Here, we explore for the first time how this approach can be applied to a specific biological system, the human kinetochore, which is a multi-protein complex involving over 100 proteins.

BackgroundThe Mitotic Spindle Assembly Checkpoint (MSAC) is an evolutionary conserved mechanism that ensures the correct segregation of chromosomes by restraining cell cycle progression from entering anaphase until all chromosomes have made proper bipolar attachments to the mitotic spindle. Its malfunction can lead to cancer.Principle FindingsWe have constructed and validated for the human MSAC mechanism an in silico dynamical model, integrating 11 proteins and complexes. The model incorporates the perspectives of three central control pathways, namely Mad1/Mad2 induced Cdc20 sequestering based on the Template Model, MCC formation, and APC inhibition. Originating from the biochemical reactions for the underlying molecular processes, non-linear ordinary differential equations for the concentrations of 11 proteins and complexes of the MSAC are derived.

We suggest a new type of modeling approach for the coarse grained, particle-based spatial simulation of combinatorially complex chemical reaction systems. In our approach molecules possess a location in the reactor as well as an orientation and geometry, while the reactions are carried out according to a list of implicitly specified reaction rules. Because the reaction rules can contain patterns for molecules, a combinatorially complex or even infinitely sized reaction network can be defined. For our implementation (based on LAMMPS), we have chosen an already existing formalism (BioNetGen) for the implicit specification of the reaction network. This compatibility allows to import existing models easily, i.e., only additional geometry data files have to be provided. Discover more details at Monitoring spindle orientation with Bashar Ibrahim.

For successful mitosis, metaphase has to be arrested until all centromeres are properly attached. The onset of anaphase, which is initiated by activating the APC, is controlled by the spindle assembly checkpoint MSAC. Mad2, which is a constitutive member of the MSAC, is supposed to inhibit the activity of the APC by sequestering away its co-activator Cdc20. Mad1 recruits Mad2 to unattached kinetochores and is compulsory for the establishment of the Mad2 and Cdc20 complexes. Recently, based on results from in vivo and in vitro studies, two biochemical models were proposed: the Template and the Exchange model. Here, we derive a mathematical description to compare the dynamical behaviour of the two models. Our simulation analysis supports the Template model. Using experimentally determined values for the model parameters, the Cdc20 concentration is reduced down to only about half.

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